Related Experiment Video
Updated: Aug 8, 2026
03:39
Preparation of Frozen Non-Human Primate Fetal Islets for Combined Single Nuclei RNA-Sequencing and ATAC-Sequencing, and Bulk Metabolomics
Published on: November 8, 2024
The Nonhuman Primate Developmental Genotype-Tissue Expression Biospecimen and Histopathology Resource
Jenna N Castro1, Samia Silva de Castro2, Rebecca L Linn3
1Division of Reproductive and Developmental Science, Oregon National Primate Research Center (ONPRC), Oregon Health & Science University (OHSU), Beaverton, Oregon, USA.
Biopreservation and Biobanking
|August 7, 2026
Summary
The Developmental Genotype-Tissue Expression (dGTEx) initiative created a comprehensive nonhuman primate (NHP) biorepository. This resource supports developmental research from prenatal stages through adulthood, aiding human health studies.
Area of Science:
- Primate genomics and developmental biology.
- Comparative genomics and translational research.
Background:
- The National Institutes of Health (NIH)-funded Developmental Genotype-Tissue Expression (dGTEx) project establishes a large nonhuman primate (NHP) sample resource.
- NHPs offer valuable translational insights into human health due to their close evolutionary relationship with humans.
Purpose of the Study:
- To create a biorepository resource using rhesus macaques (Macaca mulatta) and common marmosets (Callithrix jacchus).
- To support genetic variation studies and disease mechanism research by providing genome and RNA sequencing data.
- To establish a histopathology resource for preserved organs, aiding in tissue type and quality confirmation.
Main Methods:
- Genome and RNA sequencing for genetic variation and gene expression analysis.
- Collection and preservation of multiple organs from various developmental stages (fetal, neonatal, infant, juvenile, adult).
- Detailed documentation of animal history, demographics, and sample metadata in a secure database.
Main Results:
- Detailed sampling sites, preservation techniques, and histology review are provided.
- Tissue type and quality are confirmed through histological analysis.
- Pathological findings are noted to ensure data integrity.
Conclusions:
- The dGTEx project provides a comprehensive resource for developmental research across the entire lifespan.
- This NHP resource facilitates prenatal and postnatal developmental investigations.
- The resource supports research from early development through to adulthood.
