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Updated: Aug 8, 2026

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
Published on: July 29, 2021
DASH Diet, Dietary Nitrate, and Nitric Oxide Bioavailability: Implications for Endothelial Dysfunction and
Ruhui Zhong1, Yuxin Guo1, Xinyi You1
1College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China.
Abstract:
Hypertension in the elderly is closely associated with vascular aging, characterized by endothelial dysfunction (ED) and a progressive decline in nitric oxide (NO) bioavailability. Age-related dysfunction of endothelial nitric oxide synthase (eNOS), compounded by increased oxidative stress, creates a vicious cycle of oxidative-nitrosative imbalance, leading to chronic inflammation and arterial stiffness. The Dietary Approaches to Stop Hypertension (DASH) diet was developed as a primary intervention to restore NO balance by protecting endogenous pathways and supplying alternative nitrate-nitrite-NO precursors. However, its cardiovascular benefits are often diminished by age-related alterations in nitrate metabolism and the extensive use of multiple medications in the elderly. Thus, while restoring balance remains a key therapeutic goal, future clinical strategies must evolve from broad dietary recommendations to more personalized, precise nutritional approaches, considering the unique physiological and pharmacological challenges faced by older adults.
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