Cerebral large vessel occlusion stroke multiomics biosample cohort (CLOMB): a multicentre cohort protocol
Zhiyuan Feng1,2, Ming Yang3, Jing Xue2
1Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Stroke and Vascular Neurology
|August 7, 2026
Summary
This study establishes a protocol for biosampling the occlusion microenvironment in acute ischemic stroke with large vessel occlusion (AIS-LVO) patients undergoing endovascular treatment. It aims to identify markers for futile recanalization and improve stroke outcomes.
Area of Science:
- Neurology
- Biomarker Discovery
- Stroke Research
Background:
- Acute ischemic stroke with large vessel occlusion (AIS-LVO) requires endovascular treatment (EVT).
- Futile recanalization occurs in nearly 40% of AIS-LVO patients post-EVT.
- Understanding the occlusion microenvironment (OME) is crucial for identifying stroke markers.
Purpose of the Study:
- To establish a standardized protocol for OME biosampling and multiomics profiling in AIS-LVO patients.
- To explore candidate biological pathways associated with stroke and treatment outcomes.
- To lay the groundwork for hypothesis-generating models to improve AIS-LVO diagnosis, classification, and patient outcomes.
Main Methods:
- The Cerebral Large Vessel Occlusion Stroke Multiomics Biosample Cohort (CLOMB) study enrolled 500 AIS-LVO patients undergoing EVT across five Chinese stroke centers.
- Collected biosamples include peripheral blood, thrombus, and intracranial blood from the occlusion site.
- Multi-omics analyses (metabolomic, proteomic, transcriptomic) were performed, with single-cell RNA sequencing on a subset.
Main Results:
- A standardized protocol for OME biosampling and multiomics profiling was successfully established.
- The study collected comprehensive clinical and multi-omics data from AIS-LVO patients.
- Analysis is ongoing to identify biological pathways and candidate biomarkers.
Conclusions:
- The CLOMB study provides a robust framework for OME research in AIS-LVO.
- This multiomics approach facilitates the discovery of novel biomarkers and pathways.
- The findings will support the development of improved diagnostic and therapeutic strategies for AIS-LVO.
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