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Cerebral large vessel occlusion stroke multiomics biosample cohort (CLOMB): a multicentre cohort protocol

Zhiyuan Feng1,2, Ming Yang3, Jing Xue2

  • 1Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Stroke and Vascular Neurology
|August 7, 2026
PubMed

Insights

This study establishes a protocol for biosampling the occlusion microenvironment in acute ischemic stroke with large vessel occlusion (AIS-LVO) patients undergoing endovascular treatment. It aims to identify markers for futile recanalization and improve stroke outcomes.

Area of Science:

  • Neurology
  • Biomarker Discovery
  • Stroke Research

Background:

  • Acute ischemic stroke with large vessel occlusion (AIS-LVO) requires endovascular treatment (EVT).
  • Futile recanalization occurs in nearly 40% of AIS-LVO patients post-EVT.
  • Understanding the occlusion microenvironment (OME) is crucial for identifying stroke markers.

Purpose of the Study:

  • To establish a standardized protocol for OME biosampling and multiomics profiling in AIS-LVO patients.
  • To explore candidate biological pathways associated with stroke and treatment outcomes.
  • To lay the groundwork for hypothesis-generating models to improve AIS-LVO diagnosis, classification, and patient outcomes.

Main Methods:

  • The Cerebral Large Vessel Occlusion Stroke Multiomics Biosample Cohort (CLOMB) study enrolled 500 AIS-LVO patients undergoing EVT across five Chinese stroke centers.
  • Collected biosamples include peripheral blood, thrombus, and intracranial blood from the occlusion site.
  • Multi-omics analyses (metabolomic, proteomic, transcriptomic) were performed, with single-cell RNA sequencing on a subset.

Main Results:

  • A standardized protocol for OME biosampling and multiomics profiling was successfully established.
  • The study collected comprehensive clinical and multi-omics data from AIS-LVO patients.
  • Analysis is ongoing to identify biological pathways and candidate biomarkers.

Conclusions:

  • The CLOMB study provides a robust framework for OME research in AIS-LVO.
  • This multiomics approach facilitates the discovery of novel biomarkers and pathways.
  • The findings will support the development of improved diagnostic and therapeutic strategies for AIS-LVO.
Abstract

Keywords:
Ischemic StrokeThrombectomy

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