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09:49
Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
Published on: October 31, 2019
Integrated nitrate bioremediation and microbial risk mitigation using Bacillus pacificus
Nitish Venkateswarlu Mogili1, Dhruv Yogeshkumar Rana2, Sainikesh Padmanabhuni2
1Department of Biotechnology, Parul Institute of Technology, Parul University, Waghodia, Vadodara, Gujarat, 391760, India. nitish.mogili35319@paruluniversity.ac.in.
Biodegradation
|August 7, 2026
Summary
Bacillus pacificus effectively removes nitrate from wastewater and its biomass aids in synthesizing silver oxide nanoparticles with antibacterial properties. This integrated bioprocess offers a sustainable solution for water purification and resource recovery.
Area of Science:
- Environmental Microbiology
- Nanotechnology
- Circular Bioeconomy
Background:
- Nitrate and pathogen contamination of water sources is a critical environmental and public health issue.
- Sustainable bioagents are needed for effective water remediation within a circular economy framework.
Purpose of the Study:
- To evaluate Bacillus pacificus for nitrate remediation and antimicrobial risk reduction.
- To explore the use of bacterial biomass for green synthesis of silver oxide nanoparticles (B-Ag2ONPs).
- To assess the efficacy of the integrated bioprocess for wastewater treatment and resource recovery.
Main Methods:
- Nitrate removal efficiency was measured at different temperatures (27°C and 37°C).
- Heterotrophic denitrification pathway was inferred from nitrite accumulation.
- Biomass was used for green synthesis of B-Ag2ONPs, characterized by DLS, FTIR, and SPR.
- Antibacterial activity of B-Ag2ONPs was tested against E. coli.
- Phytotoxicity of treated wastewater was assessed on wheat seeds.
Main Results:
- Bacillus pacificus achieved high nitrate removal efficiencies (up to 85.7%).
- Green synthesized B-Ag2ONPs were spherical (15-20 nm) with a negative surface charge (-2.17 mV).
- B-Ag2ONPs demonstrated significant antibacterial activity against E. coli (MIC 17.52 mg/L).
- Treated wastewater showed reduced phytotoxicity.
Conclusions:
- Bacillus pacificus is a potent bioagent for nitrate remediation.
- The integrated bioprocess facilitates resource recovery through B-Ag2ONP synthesis.
- This approach offers a sustainable solution for water quality improvement and aligns with SDG 3 and SDG 6.
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