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Updated: Aug 9, 2026
08:37
Light-Controlled Fermentations for Microbial Chemical and Protein Production
Published on: March 22, 2022
Metabolic Engineering in Microbial System for Riboflavin Production: A Comprehensive Review
Ananya Rana1, Neetu Kumra Taneja1, Namita Singh2
1Department of Interdisciplinary Sciences, National Institute of Food Technology Entrepreneurship and Management, Sonipat, Haryana 131028 India.
Indian Journal of Microbiology
|August 8, 2026
Summary
Metabolic engineering has transformed vitamin B2 (riboflavin) production using microbial fermentation. Advanced techniques significantly boost riboflavin yield, improving efficiency and sustainability for essential nutrient supply.
Area of Science:
- Biotechnology and Metabolic Engineering
- Microbial Fermentation
- Nutritional Science
Background:
- Vitamin B2 (riboflavin) is vital for cellular metabolism and widely used in various industries.
- Traditional chemical synthesis is being replaced by sustainable microbial fermentation for riboflavin production.
Purpose of the Study:
- To review biotechnological methods for enhancing riboflavin production.
- To ensure a consistent supply of riboflavin for human and animal nutrition.
Main Methods:
- Engineering microorganisms like *A. gossypii*, *Candida famata*, and *Bacillus subtilis*.
- Reprogramming purine metabolic networks.
- Mutating key enzymes and relieving regulatory constraints.
Main Results:
- Significant improvements in riboflavin yield at an industrial scale.
- Enhanced efficiency, cost-effectiveness, and sustainability of production.
- Successful application of metabolic engineering strategies.
Conclusions:
- Metabolic engineering offers a sustainable and efficient approach to industrial riboflavin production.
- These advancements are crucial for meeting the global demand for riboflavin.
- The review serves as a key reference for researchers and industry professionals.
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