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Updated: Aug 12, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
Published on: February 24, 2015
Click-code-seq protocol for genome-wide single-nucleotide-resolution mapping of major types of DNA modifications
Lucie Lefevre1, Emilie A Aghajani1, Navnit K Singh1
1Department of Health Sciences and Technology, ETH Zurich, 8093 Zurich, Switzerland.
None:
DNA modifications are central to carcinogenesis, chemotherapy drug action, neurodegeneration, and aging. Here, we present a protocol for click-code-seq, a next-generation sequencing technique for genome-wide single-nucleotide-resolution mapping of major types of DNA modifications: oxidized guanines and abasic sites. We describe steps for genomic DNA extraction and library preparation, including enzymatic modification conversion and click-chemistry-based tagging. We then detail procedures for data analysis using the associated computational pipeline Click-pipe. For complete details on the use and execution of this protocol, please refer to Takhaveev et al.1.
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