Related Experiment Video
Updated: Aug 12, 2026
Dissection of Hippocampal Dentate Gyrus from Adult Mouse
Published on: November 18, 2009
Transcriptomic analysis of Perirhinal and Lateral Entorhinal cortex regions in mice
Beatriz B Aoyama1,2, Saúl Lindo-Samanamud1,2, João P D Machado1,2
1Laboratory of Electrophysiology, Neurobiology and Behaviour, Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas, Rua Monteiro Lobato, 255-Bloco J-1 piso-Sl 14, 13083-862, Campinas, São Paulo, Brazil.
Gene expression differences were minimal within the perirhinal cortex (PER) but significant between PER and lateral entorhinal cortex (LEC) subregions. PER shows higher nutritional sensitivity genes, while LEC subregions differ in axonal guidance and neurotransmission genes.
Area of Science:
- Neuroscience
- Molecular Biology
- Genomics
Background:
- The perirhinal cortex (PER) is vital for object recognition memory.
- The lateral entorhinal cortex (LEC) encodes object, event, and contextual information.
- Limited gene expression data exists for these distinct cortical subregions.
Purpose of the Study:
- To perform transcriptome analysis of PER (A35, A36) and LEC (DLE, VIE) subregions in mice.
- To identify molecular differences between PER and LEC subregions.
- To uncover unique gene expression profiles within these memory-associated brain areas.
Main Methods:
- Laser microdissection of specific brain regions (A35, A36, DLE, VIE).
- RNA sequencing for transcriptome-wide gene expression analysis.
- Comparative analysis of gene expression data between PER and LEC subregions.
Main Results:
- No significant gene expression differences were found between PER subregions A35 and A36.
- Substantial molecular divergence was observed between PER and LEC subregions.
- PER showed higher expression of nutritional state sensitivity genes compared to LEC.
- The DLE region exhibited elevated expression of genes related to axonal guidance and glutamatergic neurotransmission compared to VIE.
Conclusions:
- PER and LEC exhibit distinct molecular signatures.
- PER subregions A35 and A36 are molecularly similar.
- Findings provide a foundation for future research into the functional roles of these cortical regions in memory.
