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Updated: Aug 12, 2026
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Humanized Mediator Release Assay as a Read-Out for Allergen Potency
Published on: June 29, 2021
Urine mast cell mediators and anaphylaxis
Dayne Voelker1,2, Thanai Pongdee1,3
1Section of Allergy and Immunology, Department of Medicine, Mayo Clinic, Rochester.
Current Opinion in Allergy and Clinical Immunology
|August 11, 2026
Summary
Urinary mast cell mediators are promising biomarkers for detecting anaphylaxis, offering an alternative to serum tryptase. Measuring these mediators in urine during an allergic reaction shows potential for improved diagnosis.
Area of Science:
- Allergy and Immunology
- Biomarker Discovery
- Clinical Diagnostics
Background:
- Anaphylaxis is a severe hypersensitivity reaction driven by mast cell degranulation.
- Serum tryptase is the current standard biomarker but has collection limitations.
- Urinary mast cell mediators are emerging as potential diagnostic tools.
Purpose of the Study:
- To review biomarker measurements of urinary mast cell mediators in anaphylaxis.
- To highlight the role of specific urinary mediators in mast cell activation.
Main Methods:
- Review of current literature on urinary mast cell mediators in anaphylaxis.
- Focus on N-methylhistamine, leukotriene E4, and 2,3-dinor-11beta-prostaglandin F2 alpha.
Main Results:
- Urinary mast cell mediators show potential as biomarkers for anaphylaxis.
- Collection during acute episodes compared to baseline is key.
Conclusions:
- Urine mast cell mediator measurements are emerging biomarkers for anaphylaxis.
- Further research is required to validate their clinical utility.
Keywords:
2,3-dinor-11beta-prostaglandin F2 alphaanaphylaxisleukotriene E4mast cellmast cell activation syndrometryptaseMore Related Videos
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