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Advancing endodontic imaging: a high-resolution comparison between CBCT and micro-CT in the danger zone
Gabriel de Toledo Telles-Araujo1, Raimundo Sales de Oliveira Neto2, Mariana Quirino Silveira Soares3
1School of Medicine, Federal University of Bahia, Salvador, Brazil.
Biomaterial Investigations in Dentistry
|August 11, 2026
Summary
Cone-beam computed tomography (CBCT) underestimates dentin thickness in mandibular molars. Accuracy varies by protocol, with high-resolution settings showing fewer errors than standard ones for precise measurements.
Area of Science:
- Dental Imaging
- Radiology
- Endodontics
Background:
- Accurate measurement of submillimetric dentin thickness is crucial in endodontic diagnosis and treatment planning.
- The 'danger zone' (DZ) in mandibular molars requires precise assessment due to its proximity to vital structures.
Purpose of the Study:
- To evaluate the accuracy of various cone-beam computed tomography (CBCT) protocols for measuring submillimetric dentin thickness in the mandibular molar danger zone (DZ).
- To compare CBCT accuracy against micro-computed tomography (micro-CT) as the gold standard.
Main Methods:
- Fifty-one extracted mandibular molars were scanned using micro-CT and two CBCT devices with Standard, High Fidelity, and High Resolution (HIRE) protocols.
- Three-dimensional image registration was performed to measure dentin thickness in the DZ of mesial canals.
Main Results:
- All CBCT protocols significantly underestimated dentin thickness compared to micro-CT (p < 0.05), with discrepancies from 4.5% to 8.6%.
- The mesiolingual canal showed greater underestimation.
- The HIRE protocol (0.125 mm voxel size) demonstrated the smallest discrepancy, while the i-CAT protocol showed the largest.
Conclusions:
- CBCT systematically underestimates dentin thickness in the mandibular molar DZ.
- CBCT is not metrically equivalent to micro-CT for submillimetric measurements.
- Measurement accuracy is influenced by acquisition parameters beyond just voxel size.

