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Updated: Aug 12, 2026
Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
Observation of glutathione intra- and extracellular membrane with large Stokes shift probe modified by long alkyl
Lin Li1, Shao-Bin Sun2, Dan Qiao3
1School of Life Sciences, Henan Province Engineering Technology Research Center of Antibody Screening and Diagnostic, Zhengzhou Normal University, Zhengzhou, No. 16, Yingcai Street, Huiji District, Zhengzhou, 450004, Henan, P. R. China. lilin29pumc@163.com.
Abstract:
A naphthylimide dye-based fluorescent probe (Nap-Mem-GSH) was synthesized for the detection of Glutathione inside and outside cell membranes. This probe carried a 2-Hydroxyquinoline fragment as a recognition site on top of a naphthalimide dye, which exhibits better selective and fluorimetric response toward Glutathione in natural media. The long alkyl chain confers a cell membrane targeting role to Nap-Mem-GSH. Therefore, the detection of intra-and extracellular glutathione inside and outside the cell membrane can be realized by Nap-Mem-GSH. Meanwhile, Nap-Mem-GSH not only has a large Stokes shift (160 nm), a low detection limit and a rapid response toward GSH, but also has the advantages of low cytotoxicity and good membrane permeability to living cells, and has been successfully applied to effectively detect and image intracellular glutathione by confocal fluorescence imaging.
