Two porous aromatic frameworks with different porous structures for high-performance aptasensing penicillin G via...

Area of Science:

• Materials Science
• Electrochemistry
• Analytical Chemistry

Background:

• Porous Aromatic Frameworks (PAFs) offer unique structures for biosensing applications.
• Developing sensitive electrochemical aptasensors is crucial for detecting contaminants like antibiotics.
• Rational design of PAFs can enhance aptamer immobilization and sensor performance.

Purpose of the Study:

• To design and synthesize novel PAFs for enhanced aptamer immobilization.
• To develop a high-performance electrochemical aptasensor for Penicillin G (PCL) detection.
• To establish a structure-property-performance correlation in PAF-based sensors.

Main Methods:

• Synthesis of two PAFs using Friedel-Crafts alkylation.
• Characterization of PAFs' porous structures and π-conjugated systems.
• Fabrication and electrochemical detection of PCL using a PAF-pyrene based aptasensor with differential pulse voltammetry (DPV).

Main Results:

• PAF-pyrene exhibited a higher specific surface area, larger pores, and a more extensive conjugated system than PAF-naphthalene.
• The PAF-pyrene based aptasensor achieved quantitative detection of PCL within a linear range of 1.0 × 10⁻⁵ to 0.1 ng mL⁻¹.
• A low limit of detection (LOD) of approximately 0.067 pg mL⁻¹ for PCL was obtained.

Conclusions:

• The developed PAFs provide an effective platform for constructing high-performance electrochemical aptasensors.
• PAF-pyrene is a promising material for sensitive and selective detection of antibiotics.
• This work advances the application of PAFs in electrical biosensing by correlating structural properties with sensor performance.