Molecular Precursor Engineering of Lignin-Derived Carbon Dots for Multicolor Fluorescence and Metal-Ion Sensing

Lignin-derived carbon dots (CDs) are promising sustainable fluorescent nanomaterials for environmental sensing, yet precise regulation of their emission behavior and ion-recognition selectivity remains challenging. Herein, a solvent-free precursor-structure-engineering strategy was developed to prepare lignin-derived CDs with tunable photoluminescence and selective metal-ion sensing. Industrial alkali lignin, lysine, and oxalic acid were used as the carbon source, nitrogen source, and carbonization promoter, respectively, while cysteine, histidine, and p-phenylenediamine were introduced as functional precursors. The resulting C-CDs, H-CDs, and P-CDs showed distinct optical and sensing properties. H-CDs exhibited the highest photoluminescence quantum yield of 50.98%, attributed to enhanced graphitic nitrogen formation and electronic conjugation. P-CDs displayed a red-shifted emission at approximately 573 nm due to extended π-conjugated domains. Moreover, C-CDs, H-CDs, and P-CDs showed preferential fluorescence responses toward Fe³⁺, Cu²⁺, and Ag⁺, with detection limits of 0.26, 0.05, and 0.11 μM, respectively. The quenching behavior was inconsistent with a dominant dynamic collisional process and was instead associated primarily with metal-surface interactions. This work clarifies the precursor-structure-property relationship and provides a sustainable route for designing lignin-derived fluorescent probes.