Related Experiment Video
Updated: Aug 14, 2026
08:03
Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
Published on: June 25, 2017
Tuber borchii Extracts Buffer Galactose-Induced Skeletal Muscle Sarcopenia in C2C12 Myotubes
Vincenzo Aiello1,2, Leonardo Lupacchini2, Manuel Belli1,2
1Department for the Promotion of Human Science and Quality of Life, San Raffaele Open University, Via di Val Cannuta, 247, 00166 Rome, Italy.
Nutrients
|August 13, 2026
Summary
Tuber borchii extracts show promise in combating sarcopenia, a muscle-wasting condition. These natural compounds enhanced muscle protein synthesis and reduced atrophy markers in cell models, suggesting potential as a nutraceutical supplement for age-related muscle decline.
Area of Science:
- Biochemistry
- Cell Biology
- Nutraceuticals
Background:
- Sarcopenia, a decline in muscle mass and strength, significantly impacts mobility and quality of life, particularly in aging populations.
- Current interventions like physical activity are not universally applicable, necessitating exploration of alternative therapeutic avenues.
- Tuber borchii extracts have demonstrated effects on proliferation pathways, prompting investigation into their role in muscle health.
Purpose of the Study:
- To investigate the biological effects of Tuber borchii extracts on an in vitro model of sarcopenic stress.
- To assess the impact of Tuber borchii extracts on protein synthesis, turnover, and cellular morphology in sarcopenic conditions.
Main Methods:
- Differentiated myotubes were exposed to D-galactose to induce sarcopenia-like cellular changes.
- The effects of Tuber borchii extracts were evaluated by measuring protein synthesis and turnover.
- Cell morphology, including thickness and cross-sectional area, was analyzed to assess protective effects.
Main Results:
- Tuber borchii extracts significantly enhanced protein synthesis and turnover in myotubes.
- Treatment with the extracts reduced the expression of sarcopenia and atrophy markers, such as MuRF1.
- Morphological analysis revealed that treated myotubes maintained greater thickness and cross-sectional area.
Conclusions:
- Tuber borchii extracts demonstrated the ability to mitigate cellular alterations associated with sarcopenia and muscle aging.
- These findings suggest the potential of Tuber borchii as a source for developing novel nutraceuticals to address age-related muscle loss.
