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Updated: Aug 14, 2026

Human Circadian Phenotyping and Diurnal Performance Testing in the Real World
Published on: April 7, 2020
Effects of Three Consecutive Days of Diurnal Intermittent Fasting on Cognitive Function, Psychomotor Vigilance, and
Hamza Baati1, Sabra Ouni1, Fida Ayachi2
1Education, Motricité, Sport et Santé (EM2S), UR15JS01, High Institute of Sport and Physical Education, University of Sfax, Sfax 3000, Tunisia.
Abstract:
Aim: While voluntary Islamic intermittent fasting (IF) of three consecutive days (mimicking practices such as Ayyam al-Bid) may be observed by Muslim athletes, its short-term effects on cognitive function and sleep quality in adolescent team-sport athletes remain unexamined. This study investigated the impact of three successive days of dawn-to-sunset IF on memory, attention, psychomotor vigilance, and subjective sleep quality in young male soccer players. Methods: A quasi-experimental, parallel-group design was employed. Thirty-five male soccer players (age: 15.6 ± 0.8 years; body mass: 55.07 ± 6.53 kg) were allocated to an IF group (n = 17) or a non-fasting control group (n = 18). The fasting protocol comprised approximately 14 consecutive hours of complete food and fluid abstinence per day (from 04:40 to 18:36, with daily variations of -3 min for the dawn and +2 min for the sunset, local time). Cognitive function was assessed using the CogniFit Cognitive Assessment Battery (CAB) and the Psychomotor Vigilance Test (PVT), and subjective well-being was quantified via the Hooper Index. Both assessment sessions were conducted between 14:00 and 17:00 to control for diurnal variation. Results: The Group × Time interaction for single-item subjective sleep quality reached nominal significance at the unadjusted threshold (Z = 2.18, p = 0.029, r = 0.37) but did not survive Bonferroni correction for the four Hooper Index comparisons (α = 0.0125); this result is therefore interpreted as hypothesis-generating only, reflecting a within-group deterioration in the control group (p < 0.05) alongside a non-significant marginal improvement in the fasting group. No statistically significant Group × Time interaction was detected for any memory subdomain (all p ≥ 0.419, r ≤ 0.14), attention component (all p > 0.176, r ≤ 0.23), or PVT parameter (all p > 0.192, η2p/r ≤ 0.22) under the present study conditions. Within-group improvements in memory and attention observed in both groups were consistent with test-retest learning effects. Conclusions: No statistically significant Group × Time interaction was detected for memory, attention-related executive functions, or psychomotor vigilance in young male soccer players under the present study conditions; the study was not designed as an equivalence or non-inferiority trial and cannot exclude effects smaller than the targeted medium magnitude, alongside a nominally divergent but statistically uncorrected sleep quality trajectory relative to non-fasting controls, which should be interpreted as hypothesis-generating pending replication. These findings provide preliminary, hypothesis-generating evidence that no statistically significant cognitive or psychomotor impairment was detected under the present study conditions; because the study was not designed as an equivalence or non-inferiority trial, compatibility between short-term voluntary IF and adolescent athletic training cannot be inferred from the absence of a significant interaction alone.