Screening and Evaluation of Quinoa Germplasm for Saline-Alkali Tolerance Based on Germination Vigor Traits

Soil salinization and alkalization caused by global climate change have become major constraints on crop production worldwide. Quinoa (Chenopodium quinoa Willd.), owing to its exceptional adaptability to adverse environmental conditions, is considered a promising crop for saline-alkali agriculture. Successful screening and evaluation of saline-alkali-tolerant germplasm at the germination stage is essential for breeding quinoa varieties with enhanced tolerance to compound saline-alkali stress. In this study, 23 quinoa accessions originating from different regions were evaluated for saline-alkali tolerance by exposing seeds to compound saline-alkali solutions at different concentrations in a Petri dish germination assay. During the germination stage, relative vigor index, germination rate, germination energy, shoot length, root length, and fresh weight were determined, and saline-alkali tolerance was assessed using multivariate statistical analyses. The germination ability and seedling growth potential of quinoa germplasm gradually declined with increasing saline-alkali stress. Based on the comprehensive saline-alkali tolerance index at the germination stage, eight accessions were identified as highly tolerant and four accessions as highly sensitive to saline-alkali stress. Among them, LL1 and Z27 exhibited outstanding tolerance and were identified as elite saline-alkali-tolerant germplasm resources. The results indicated that vigor index and fresh weight at the germination stage can serve as key parameters for evaluating saline-alkali tolerance in quinoa germplasm. Overall, this study provides valuable germplasm resources and a practical basis for breeding new quinoa varieties with enhanced saline-alkali tolerance, as well as for the identification and utilization of stress-resistance-related genes in quinoa.