Guanidinoacetic Acid as a Dietary Additive for Lambs: A Meta-Analysis on Performance, Antioxidant Status, Nutrient...

This study aimed to evaluate the effects of dietary supplementation with guanidinoacetic acid (GAA) on growth performance, antioxidant status, nutrient digestibility, ruminal fermentation, and meat quality in lambs through a meta-analysis. The electronic databases Scopus, Web of Science, ScienceDirect, and Google Scholar were used to identify the articles required for the meta-analysis using the Preferred Reporting Elements for Systematic Reviews and Meta-Analyses (PRISMA) methodology. The data used in this meta-analysis were obtained from 12 peer-reviewed English-language articles published in the last decade (January 2016 to April 2026). All data were analyzed using random-effects models. Dietary supplementation with GAA decreased the feed conversion ratio (p < 0.05) and increased (p < 0.05) dry matter intake, average daily gain, carcass weight and yield, and Longissimus dorsi muscle area. Dietary supplementation with GAA decreased (p < 0.001) serum malondialdehyde concentration and increased (p ≤ 0.05) serum concentrations of superoxide dismutase, catalase, glutathione peroxidase, and total antioxidant capacity. Dietary supplementation with GAA decreased (p < 0.001) ruminal pH and increased (p < 0.05) the digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber, as well as the ruminal concentrations of total volatile fatty acids, acetate, and butyrate. Dietary supplementation with GAA decreased (p < 0.001) drip loss in meat and increased (p < 0.001) meat pH and meat protein content. In conclusion, guanidinoacetic acid can be used as a dietary additive to improve growth performance, antioxidant status, nutrient digestibility, ruminal fermentation, and meat quality in lambs.