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Whole-Transcriptome Sequencing Analysis of Fowl Adenovirus Serotype 4 Infection in LMH Cells
Areayi Haiyilati1,2,3,4, Xinrui Wang1,2,3,4, Xin Miao1,2,3,4
1College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China.
International Journal of Molecular Sciences
|August 13, 2026
Summary
Fowl adenovirus serotype 4 (FAdV-4) causes avian hepatitis-hydropericardium syndrome (HHS). This study reveals extensive transcriptomic changes in LMH cells upon FAdV-4 infection, uncovering key virus-host interactions.
Area of Science:
- Veterinary Virology
- Molecular Biology
- Transcriptomics
Background:
- Fowl adenovirus serotype 4 (FAdV-4) is a significant poultry pathogen causing avian hepatitis-hydropericardium syndrome (HHS).
- Understanding FAdV-4's impact on host cells is crucial for mitigating poultry industry losses.
Purpose of the Study:
- To investigate virus-host interactions and transcriptomic alterations in LMH cells infected with FAdV-4.
- To elucidate the molecular mechanisms underlying FAdV-4 pathogenesis.
Main Methods:
- LMH cells were infected with FAdV-4 (MOI=1) for 24 hours.
- Whole-transcriptome sequencing was performed, followed by differential expression analysis.
- Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and network analyses were conducted.
Main Results:
- Identified 8292 differentially expressed mRNAs, 80 miRNAs, 3263 lncRNAs, and 52 circRNAs.
- Functional enrichment analyses revealed significant pathways involved in host response.
- lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA regulatory networks were constructed.
Conclusions:
- FAdV-4 infection induces widespread transcriptomic changes in host cells.
- This study provides insights into FAdV-4 pathogenesis and potential therapeutic targets.
