Strategies for Multiplexing Plasmonic Biosensing

Area of Science:

• Plasmonic biosensing
• Multiplexed optical biosensing
• Nanoplasmonic biosensing

Background:

• Plasmonic biosensors provide sensitive, label-free detection of biomolecular interactions.
• Multiplexing strategies are crucial for comprehensive molecular profiling and simultaneous analysis.
• Plasmonic sensors offer advantages over other optical methods like fluorescence and interferometry.

Purpose of the Study:

• To examine multiplexing strategies in plasmonic biosensing across spatial, spectral, and temporal dimensions.
• To critically discuss current multiplexing techniques including SPR, LSPR, and SERS.
• To highlight applications and challenges in plasmonic biosensing.

Main Methods:

• Review of plasmonic biosensing techniques (SPR, LSPR, SERS).
• Analysis of multiplexing dimensions (spatial, spectral, temporal) and hybrid approaches.
• Discussion of surface biofunctionalisation, antifouling, and molecular recognition.

Main Results:

• Plasmonic biosensors are attractive for label-free, real-time interaction monitoring.
• Multiplexing strategies are being advanced through various dimensions and hybrid combinations.
• Applications span diagnostics, liquid biopsy, glycoprofiling, and environmental analysis.

Conclusions:

• The future of multiplexed plasmonic biosensing relies on robust, standardized analytical systems.
• Addressing challenges in reproducibility, standardization, and data interpretation is key for clinical translation.
• Plasmonic biosensing holds significant potential when integrated into standardized platforms.