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Antibacterial Efficacy of Artemisia Extract and White Vinegar as Disinfectants for Acrylic Prostheses Fabricated
Mariette Droubi1, Joul Kassis2, Alaa Salloum1
1Department of Removable Prosthodontics, Faculty of Dentistry, Damascus University, Damascus, Syria, damascusuniversity.edu.sy.
Objective:
To evaluate the antibacterial efficacy of Artemisia herba-alba extract and white vinegar as natural disinfectants against Staphylococcus aureus on computer-aided design and computer-aided manufacturing (CAD/CAM)-milled and heat-polymerized denture base resins.
Materials And Methods:
Sixty acrylic resin blocks were prepared and divided into two main groups (n = 30 each): CAD/CAM-milled and heat-polymerized acrylic resin. The blocks were contaminated with Staphylococcus aureus isolated from a patient with denture-related stomatitis and immersed for 60 min in one of three solutions: Artemisia herba-alba extract, white vinegar, or distilled water (negative control). Antibacterial efficacy was assessed by quantifying colony forming units (CFUs) using the viable count method.
Results:
A Kruskal-Wallis test revealed that both Artemisia herba-alba extract and white vinegar significantly lowered CFU counts relative to the control (p < 0.05) across both material groups. Notably, the Artemisia herba-alba extract exhibited superior antibacterial efficacy compared to white vinegar, yielding log reductions of 4.11 and 3.42 in heat-polymerized and CAD/CAM acrylic specimens, respectively. In contrast, white vinegar produced more modest log reductions of 1.81 and 1.20, respectively. Under the studied conditions, heat-polymerized acrylic consistently demonstrated slightly higher bacterial reduction than CAD/CAM acrylic.
Conclusion:
Both Artemisia herba-alba extract and white vinegar demonstrated antibacterial activity against Staphylococcus aureus on removable denture base materials, with the extract demonstrating superior potency. While heat-polymerized acrylic showed slightly higher bacterial reduction than CAD/CAM acrylic, both material types responded positively to the tested disinfecting agents.
Significance:
This study demonstrates that Artemisia herba-alba extract and white vinegar are effective natural disinfectants against Staphylococcus aureus associated with denture materials. It supports their use as low-cost natural alternatives for managing denture stomatitis and improving denture hygiene. Alternatively, it highlights their potential as low-cost natural alternatives for improving denture hygiene and warrants further investigation regarding clinical applicability and long-term safety.
