Related Experiment Video
Updated: Aug 14, 2026
07:52
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
Published on: November 2, 2020
In vivo CRISPR editing for cancer immunotherapy
Cole W Christopher1,2, Xiaoyu Zhou1,2
1Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia, PA, United States.
Frontiers in Immunology
|August 13, 2026
Summary
Clustered regularly interspaced short palindromic repeats (CRISPR) gene editing shows promise for cancer immunotherapy by targeting tumors and immune cells. Further research is needed to optimize CRISPR platforms for safe and effective clinical translation.
Area of Science:
- Oncology
- Immunology
- Gene Editing
Background:
- Cancer immunotherapy offers significant benefits but requires broader patient applicability.
- Clustered regularly interspaced short palindromic repeats (CRISPR)-based gene editing is advancing towards therapeutic applications.
Purpose of the Study:
- To review recent advancements in applying CRISPR systems *in vivo* for cancer immunotherapy.
- To highlight strategies targeting cancer cells, the tumor microenvironment, and immune cell engineering.
Main Methods:
- Review of *in vivo* CRISPR applications in cancer immunotherapy.
- Focus on CRISPR strategies for direct cancer cell and tumor microenvironment modification.
- Analysis of CRISPR-based immune cell engineering approaches.
Main Results:
- CRISPR systems are being explored to target cancer cells and the tumor microenvironment *in vivo*.
- Engineering immune cells using CRISPR shows potential for enhancing antitumor immunity.
- Novel CRISPR platforms enable multiplexed editing, impacting antitumor responses.
Conclusions:
- CRISPR-based *in vivo* cancer immunotherapy is a rapidly evolving field.
- Early-stage CRISPR platforms show promise but require further investigation for clinical translation.
- Future research should integrate functional genomics and optimize delivery systems for efficacy and safety.
Related Concept Videos
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
01:28CRISPR/Cas9 Genome Editing
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
02:53CRISPR and crRNAs
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.