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Lactobacillus paragasseri OLL2716 Promotes Ghrelin Expression in Gastric Cells
Aoi Fujieda1, Naruomi Yamada2, Akika Nagira2
1Health Science Research Unit, R&D Division, Meiji Co., Ltd., 1-29-1 Nanakuni, Hachioji, Tokyo, 192-0919, Japan. aoi.fujieda@meiji.com.
Probiotics and Antimicrobial Proteins
|August 13, 2026
Summary
LG21 yogurt, containing Lactobacillus paragasseri OLL2716, increases ghrelin production in gastric cells. This strain-specific effect, observed in vitro, suggests a role for LG21 in modulating gastric function and hormone secretion.
Area of Science:
- Gastroenterology
- Microbiology
- Endocrinology
Background:
- Yogurt with Lactobacillus paragasseri OLL2716 (LG21) may improve gastric emptying and autonomic nervous system (ANS) activity.
- Ghrelin, a stomach-produced hormone, influences gastric motility, acid secretion, and autonomic function.
Purpose of the Study:
- To investigate the effect of LG21 yogurt on ghrelin-related responses in human gastric cells.
- To determine if LG21 yogurt influences ghrelin messenger RNA (mRNA) expression and secretion.
Main Methods:
- MKN45 human gastric adenocarcinoma cells were treated with live or heat-treated LG21 cells, LG21 culture supernatant, or LG21-free yogurt.
- Ghrelin mRNA expression was quantified using RT-qPCR.
- Ghrelin secretion was measured using enzyme-linked immunosorbent assay (ELISA).
- Pharmacological experiments explored signaling pathways involved in the ghrelin response.
Main Results:
- Both LG21 cells and their supernatant significantly increased ghrelin mRNA expression in MKN45 cells.
- LG21 cells also significantly increased ghrelin secretion.
- LG21 yogurt induced a faster increase in ghrelin mRNA compared to LG21-free yogurt.
- The effect on ghrelin mRNA expression was strain-dependent, with LG21 showing a significant effect while the L. paragasseri type strain did not.
- Prostaglandin E2-EP4 signaling was implicated in the ghrelin response.
Conclusions:
- LG21 yogurt modulates ghrelin production in gastric cells in a strain-dependent manner.
- Both LG21 bacteria and their metabolites contribute to increased ghrelin expression and secretion.
- These findings suggest a potential mechanism for LG21 yogurt in influencing gastric function via ghrelin modulation.
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