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Updated: Aug 15, 2026

Enrichment of mRNA and Bisulfite-mRNA Library Preparation for Next-Generation Sequencing
06:57

Enrichment of mRNA and Bisulfite-mRNA Library Preparation for Next-Generation Sequencing

Published on: July 7, 2023

RNA methyltransferase NSUN5 promotes oral squamous cell carcinoma proliferation and migration by enhancing YAP1

Huiqi Bao1, Jun Yuan2, Baixue Zhai2

  • 1Department of Stomatology, Songjiang Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 201600, China.

Odontology
|August 13, 2026
PubMed

Insights

NSUN5, a key methyltransferase, is upregulated in oral squamous cell carcinoma (OSCC) and promotes tumor progression by stabilizing YAP1 mRNA. This identifies a novel NSUN5/YAP1 pathway in OSCC development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • m5C modifications are crucial in oral squamous cell carcinoma (OSCC) development.
  • NSUN5, a methyltransferase, catalyzes mRNA m5C modification, but its role in OSCC is unknown.

Purpose of the Study:

  • To investigate the oncogenic role and molecular mechanisms of NSUN5 in OSCC progression.
  • To explore the NSUN5/YAP1 pathway in OSCC.

Main Methods:

  • Analysis of NSUN5 expression in 34 OSCC tissues and 7 cell lines using qRT-PCR, Western blotting, and IHC.
  • Evaluation of NSUN5's impact on OSCC cell proliferation, migration, and invasion via CCK8, colony formation, and wound healing assays.
  • Detection of YAP1 expression and m5C modification in NSUN5-silenced cells.

Main Results:

  • NSUN5 was upregulated in OSCC tissues and correlated with poor prognosis.
  • NSUN5 promoted OSCC cell proliferation, migration, invasion, and tumor growth in vivo.
  • NSUN5 stabilizes YAP1 mRNA via m5C modification, increasing YAP1 expression.

Conclusions:

  • NSUN5 acts as an oncogene in OSCC progression.
  • The NSUN5/YAP1 axis is a novel pathway regulating YAP1 expression in OSCC.
  • Targeting NSUN5 may offer a therapeutic strategy for OSCC.
Keywords:
Hippo pathwayNSUN5Oral squamous cell carcinomaYAP1m5C modification

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