Related Experiment Video
Updated: Aug 15, 2026

Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples
Published on: March 26, 2021
Comparative analysis of Rhipicephalus (Boophilus) microplus by scanning electron microscopy with and without gold
Geziella Aurea Aparecida Damasceno Souza1, Fernando Veloso Caldeira Barcellos1, Juliana Pimenta Cruz1
1Campus Universitário Professor Darcy Ribeiro - Av. Prof. Rui Braga, s/n - Vila Mauricéia, Montes Claros, MG 39401-089, Brazil.
Gold coating for Scanning Electron Microscopy (SEM) of Rhipicephalus (Boophilus) microplus larvae can obscure ultra-structural details. Uncoated samples offer richer fine details, making them preferable for certain Rhipicephalus (Boophilus) microplus studies.
Area of Science:
- Veterinary Parasitology
- Microscopy Techniques
- Entomology
Background:
- Rhipicephalus (Boophilus) microplus is a significant cattle ectoparasite causing economic losses and disease transmission globally.
- Scanning Electron Microscopy (SEM) is valuable for studying parasite morphology.
- Sample preparation, specifically gold coating, is debated for its impact on SEM image quality.
Purpose of the Study:
- To compare the effects of gold coating duration on SEM imaging of Rhipicephalus (Boophilus) microplus larvae.
- To evaluate the trade-offs between contrast enhancement and artifact introduction during SEM sample preparation.
Main Methods:
- Rhipicephalus (Boophilus) microplus larvae (21 days post-hatching) were prepared for SEM.
- Samples were analyzed with SEM both with and without gold coating (20s and 40s).
- Images were visually compared to assess morphological detail and image quality.
Main Results:
- Gold coating for 20s provided balanced contrast but introduced artifacts from metal agglomeration, obscuring fine details.
- Gold coating for 40s resulted in surface cracking and structural disruption.
- Uncoated samples exhibited high contrast and revealed rich fine morphological details.
Conclusions:
- The decision to gold coat Rhipicephalus (Boophilus) microplus larvae for SEM depends on research objectives, alternative conductive methods, and resource availability.
- Uncoated samples may be superior for detailed morphological studies of Rhipicephalus (Boophilus) microplus.
- Careful consideration of coating parameters is crucial to optimize SEM imaging of ectoparasites.
More Related Videos
10:59Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies
Published on: June 1, 2019
07:00Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
Published on: August 5, 2021
Related Concept Videos
Immunogold Electron Microscopy
Preparation of Samples for Electron Microscopy
Scanning Electron Microscopy
Fundamental Principles
Accelerated...
Overview of Electron Microscopy
Overview of Microscopy Techniques