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Updated: Aug 15, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
Integrated soft X-ray/visible fluorescence microscope using divided-pupil reflective optics for correlative cellular
Researchers developed a new microscope for real-time imaging of living cells. This advanced system integrates soft X-ray and fluorescence microscopy, enabling detailed visualization of cellular dynamics without mechanical switching.
Area of Science:
- Cellular biology
- Microscopy
- Biophysics
Background:
- Observing dynamic cellular processes in real-time is essential for understanding cell function.
- Existing microscopy techniques often lack the resolution or correlative capabilities needed for comprehensive live-cell analysis.
Purpose of the Study:
- To develop an integrated correlative microscope for simultaneous soft X-ray and visible fluorescence imaging of living cells.
- To overcome limitations of mechanical switching in correlative microscopy systems.
Main Methods:
- Development of a full-field microscope utilizing divided-pupil reflective optics for a common field of view.
- Generation of a water-window soft X-ray beam from a soft X-ray free-electron laser (SXFEL).
- Sequential fluorescence and soft X-ray imaging of chemically fixed and living cells.
Main Results:
- Demonstrated successful localization of fluorescent agents and visualization of structural details in fixed cells.
- Achieved correlative visualization of living cells expressing a cell cycle fluorescence marker.
- Validated the performance of the mechanically switch-free integrated microscope system.
Conclusions:
- The developed microscope enables advanced correlative imaging of living cells with high resolution.
- The mechanically switch-free design provides a versatile platform for future simultaneous correlative imaging studies.
- This technology advances the study of dynamic cellular processes in real-time.
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