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Fiber-optic SPR sensor with layer-by-layer hydrogel-entrapped enzymes for specific glyceryl tributyrate detection
Optics Express
|August 14, 2026
Summary
A novel Ag-coated no-core fiber SPR sensor offers sensitive and stable detection of glyceryl tributyrate. This hydrogel-encapsulated lipase platform enhances enzyme activity and sensor longevity for health and food safety applications.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Materials Science
Background:
- Conventional enzymatic assays for glyceryl tributyrate face challenges like lipase deactivation and signal drift.
- There is a need for sensitive, stable, and interference-resistant detection platforms.
Purpose of the Study:
- To develop a robust surface plasmon resonance (SPR) sensor for quantitative glyceryl tributyrate detection.
- To utilize a layer-by-layer (LbL) assembled hydrogel biointerface encapsulating lipase for enhanced sensor performance.
Main Methods:
- Fabrication of an Ag-coated no-core fiber (NCF) SPR sensor.
- Assembly of a hydrogel biointerface using LbL technique to immobilize lipase.
- Optimization of LbL layers and evaluation of sensor performance (sensitivity, stability, selectivity).
Main Results:
- A three-layer LbL configuration demonstrated optimal performance.
- The sensor achieved a sensitivity of 2.40 nm/mM for glyceryl tributyrate detection within the 0.5-10 mmol/L range.
- The hydrogel interface preserved enzyme activity, enhanced stability, and reduced interference.
Conclusions:
- The proposed fiber-optic SPR sensor provides a reliable, low-cost method for glyceryl tributyrate detection.
- The sensor exhibits excellent operational stability and reusability.
- This platform is suitable for health monitoring and food safety screening.
