SeCaT-meter 600: Prism-based dual channel optical density meter with self-calibration

Area of Science:

• Biotechnology
• Bioprocess Engineering
• Instrumentation

Background:

• Accurate optical density (OD) monitoring is crucial for controlling microbial fermentations and understanding cell growth dynamics.
• Traditional OD600 measurement methods using UV/Vis spectrometers are costly and unsuitable for resource-limited or field applications.
• Off-line measurements are common in labs due to high on-line device costs, limiting real-time process control.

Purpose of the Study:

• To design and develop a low-cost, self-calibrating photometer for precise OD600 measurements.
• To provide a robust and portable solution for bioprocess monitoring in diverse settings.
• To overcome the limitations of expensive and complex commercial spectrophotometers.

Main Methods:

• Utilized widely available electronic components and 3D printing technology to construct the photometer.
• Integrated a self-calibration mechanism for accurate and reliable measurements.
• Focused on affordability, portability, and ease of use for laboratory and field applications.

Main Results:

• The developed photometer is capable of precise OD600 measurements for bioprocess monitoring.
• The device offers a cost-effective alternative to conventional spectrophotometers.
• The design facilitates portability and ease of use, suitable for various experimental scales and field deployment.

Conclusions:

• The self-calibrating photometer presents a viable, affordable solution for accurate OD600 monitoring in microbial fermentations.
• This innovation can significantly benefit resource-limited laboratories and field-based bioprocess applications.
• The device enhances accessibility to essential bioprocess monitoring tools.