Insights into glucomoringin biosynthesis in moringa sprouts through integrated transcriptomic and metabolomic...

Glucomoringin (GMG) is a distinctive glucosinolate rich in moringa seeds and sprouts. GMG has gained considerable attention as a precursor of moringin (RBITC), a unique isothiocyanate with anticancer potential. However, studies on GMG biosynthesis in moringa sprouts remain scarce, highlighting the needs for investigation. In this study, a tyrosine-associated GMG biosynthetic pathway was proposed based on transcript annotation and metabolite prediction. The candidate genes potentially associated with GMG biosynthesis were tentatively identified, such as CYP79A1, CYP83B1, SUR1, UGT74B1, and SOT16. Two additional genes TGG4 and TGG5 were tentatively identified as candidates possibly involved in the bioconversion of GMG to RBITC. These findings offer valuable insights into GMG biosynthesis and may facilitate the development of strategies to enhance GMG production in moringa sprouts.