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The use of multi-species probiotic-derived vesicles to encapsulate luteolin for preventing colitis in a mouse model
Shulin Hou1, Haishan Yang1,2, Qian Wang1
1Department of Biochemistry and Molecular Biology, College of Basic Medicine, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, MOE Key Laboratory of Coal Environmental Pathogenicity and Prevention, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
Abstract:
Probiotic-derived membrane vesicles (MVs) offer advantages over live probiotics, including lower immunogenicity and enhanced bioavailability of dietary compounds. However, their composition and function depend on culture conditions. In this study, MVs from a single probiotic strain (S-MVs) were compared with those from co-cultured multi-species probiotics (M-MVs). M-MVs showed superior radical scavenging ability due to characteristic antioxidant proteins. Luteolin (Lut), a hydrophobic flavonoid, was loaded into M-MVs to generate Lut-MVs. Compared with Lut or M-MVs alone, Lut-MVs had enhanced water solubility, gastrointestinal stability, and antioxidative capacity. In a dextran sulfate sodium (DSS)-induced mouse colitis model, Lut-MVs prevented colitis by alleviating oxidative damage, improving intestinal barrier function, reducing inflammatory responses, and regulating colonic microbial balance. This work provides a method for preparing probiotic-derived vesicles and highlights their potential as carriers for hydrophobic drugs in treating colon diseases.
Insights
Probiotic membrane vesicles (MVs) loaded with luteolin (Lut-MVs) show enhanced antioxidant capacity. Lut-MVs effectively treat colitis in mice by improving gut health and reducing inflammation.
Area of Science:
- Microbiology
- Biotechnology
- Pharmacology
Background:
- Probiotic-derived membrane vesicles (MVs) offer advantages over live probiotics.
- MV composition and function are influenced by culture conditions.
- Comparing single-strain MVs (S-MVs) with multi-species MVs (M-MVs) is crucial.
Purpose of the Study:
- To compare MVs from single-strain and multi-species probiotics.
- To develop luteolin-loaded M-MVs (Lut-MVs) for enhanced therapeutic delivery.
- To evaluate the efficacy of Lut-MVs in a mouse model of colitis.
Main Methods:
- Cultured single-strain and multi-species probiotics to produce MVs.
- Loaded hydrophobic luteolin into M-MVs.
- Assessed physicochemical properties and antioxidant capacity of MVs.
- Induced colitis in mice using dextran sulfate sodium (DSS).
- Administered Lut-MVs to DSS-induced colitis model mice.
Main Results:
- M-MVs exhibited superior radical scavenging activity compared to S-MVs.
- Lut-MVs demonstrated improved water solubility, gastrointestinal stability, and antioxidative capacity.
- Lut-MVs treatment in mice prevented colitis by reducing oxidative damage and inflammation.
- Lut-MVs improved intestinal barrier function and regulated colonic microbial balance.
Conclusions:
- Probiotic-derived MVs can be engineered for enhanced therapeutic properties.
- Lut-MVs show significant potential for treating colon diseases like colitis.
- This study provides a novel method for preparing drug-loaded MVs.
