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Updated: Aug 19, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Amino-functionalized hydrogel immobilized microbial consortia to reduce the dissemination risks of antibiotic
Yongxin Guo1, Jiaoyang Ji1, Xianfeng Lin2
1Jilin Provincial Key Laboratory of Environmental Ecology in Black Soils, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Abstract:
Antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) have posed an environmental threat to soil due to the excessive utilization of antibiotics. Traditional material-biological remediation strategies were limited by the difficulty of colonizing functional bacteria and insufficient removal efficiency of ARB and ARGs. Here, a functionalized carboxymethyl chitosan hydrogel with functional microbial consortia (NCMC + mc) remediation strategy was employed to reduce the dissemination risks of ARGs in soil and improve soil health. The NCMC + mc treatment reduced the levels of targeted ARGs in soil by 58.05 %-94.24 %. The crop cultivation and earthworm breeding experiments conducted in the restored soil demonstrated the effectiveness of NCMC + mc in reducing the spread of ARGs. The targeted ARGs in crops were reduced by 77.17 %-90.80 %, and those in earthworms by 52.13 %-94.71 %. Random Forest analysis predicted that Acidimicrobia was the main host or spreading bacteria for ARGs. The effectiveness of NCMC + mc system was further confirmed by TOPSIS evaluation model. This study provides an effective remediation strategy for reducing the dissemination of ARGs to improve soil health.
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