A Mouse Model for Fusobacterium nucleatum-Mediated Experimental Periodontitis

Fusobacterium nucleatum is a Gram-negative, anaerobic bacterium that naturally colonizes the human oral cavity and is recognized as an etiological agent in both local and systemic diseases, including periodontitis, preterm birth, and colorectal cancer. To advance research into the systemic impact of oral-origin F. nucleatum-associated pathologies, it is crucial to optimize the use of animal models. In this study, we present a detailed protocol for establishing a mouse model of experimental periodontitis through oral inoculation of bacteria, including F. nucleatum. This model provides a valuable platform for investigating the pathogenic mechanisms and systemic consequences of oral bacterial infections.