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miR-379-5p serves as a biomarker and regulates sepsis-associated acute kidney injury via targeting EDN1

Xiaoyu Wang1, Hongdong Li1, Xianfeng Yu2

  • 1Department of Nephrology, The 960th Hospital of the PLA Joint Logistics Support Force, Jinan, Shandong Province, 255000, China.

BMC Immunology
|August 19, 2026
PubMed
Abstract

Insights

Low serum miR-379-5p levels can help diagnose sepsis-associated acute kidney injury (SA-AKI) and predict patient survival. MiR-379-5p protects kidney cells from injury by targeting EDN1.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nephrology

Background:

  • Sepsis-associated acute kidney injury (SA-AKI) is a critical complication in sepsis patients.
  • MicroRNAs (miRNAs) play a role in the development of SA-AKI.
  • Investigating specific miRNAs like miR-379-5p is crucial for understanding SA-AKI pathogenesis.

Purpose of the Study:

  • To evaluate the clinical significance of serum miR-379-5p in diagnosing SA-AKI.
  • To assess the correlation between miR-379-5p levels and kidney injury markers.
  • To explore the protective mechanism of miR-379-5p in lipopolysaccharide (LPS)-induced kidney injury.

Main Methods:

  • Serum miR-379-5p levels were quantified in sepsis and SA-AKI patients.
  • Correlation analyses were performed between miR-379-5p and clinical parameters (Scr, BUN, Cys-C, KIM-1, CRP, PCT, WBC).
  • An in vitro model using LPS-stimulated HK-2 cells was employed to study miR-379-5p's function and its interaction with EDN1.

Main Results:

  • Lower serum miR-379-5p expression was observed in SA-AKI patients compared to sepsis patients.
  • miR-379-5p levels negatively correlated with kidney injury markers and inflammatory indicators.
  • Overexpression of miR-379-5p mitigated LPS-induced HK-2 cell damage and inflammation by targeting EDN1.

Conclusions:

  • Downregulated serum miR-379-5p is a potential biomarker for SA-AKI diagnosis and predicting 28-day survival.
  • miR-379-5p exerts a protective effect against LPS-induced kidney injury in HK-2 cells.
  • The protective mechanism involves targeting and inhibiting the expression of EDN1.
Keywords:
BiomarkerDiagnosisEDN1Sepsis-associated acute kidney injurymiR-379-5p

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