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Updated: Aug 21, 2026
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
Published on: January 30, 2017
Development of a rapid, sensitive and visual reverse transcription recombinase-aided amplification coupled with
Yanhong Liu1, Heyuan Geng2, Yuan Wang3
1QT Biotech Co., Ltd, Wuxi, China.
Introduction:
Influenza B virus (IBV) is a major etiological agent of seasonal influenza, imposing a significant global health burden. Rapid and accurate detection is crucial for epidemic control, but conventional methods are limited by complex equipment or long turnaround times.
Methods:
Herein, we developed a reverse transcription recombinase-aided amplification assay combined with lateral flow dipstick (RT-RAA-LFD) for specific IBV detection, targeting the conserved ns gene.
Results:
Following primer screening, the optimal primer pair F2/R4 was identified. The assay was optimized to react at 39°C for 20 min with a detection volume of 15 μL. Analytical validation demonstrated high sensitivity (50 copies/mL) and no nonspecific cross-reactivity with other common respiratory viruses, including influenza A virus, SARS-CoV-2, parainfluenza virus and adenovirus. Clinical performance evaluation revealed 100% concordance with the gold standard PCR assay.
Discussion:
Collectively, the established RT-RAA-LFD assay is rapid, specific, sensitive and easy to operate without relying on sophisticated instruments, making it a promising tool for point-of-care testing (POCT) and field surveillance of IBV infections.
