Related Experiment Video

Updated: Aug 21, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Upregulation of ITM2A by p53 activation modulates p53 function through physical interaction

Sim Namkoong1,2, Minsu Jang1, Jeong In Lee2

  • 1Division of Biological Science and Technology, Yonsei University, Wonju, Republic of Korea.

Cell Cycle (Georgetown, Tex.)
|August 19, 2026
PubMed

Insights

The tumor suppressor p53 regulates ITM2A, which then inhibits p53 activity, forming a feedback loop. Reduced ITM2A levels correlate with poor survival in cancers with faulty p53.

Area of Science:

  • Molecular Biology
  • Cancer Biology
  • Biochemistry

Background:

  • The tumor suppressor p53 is critical for preventing cancer.
  • p53 dysfunction is common in many cancers.
  • A novel interaction between p53 and ITM2A was investigated.

Purpose of the Study:

  • To identify and characterize the regulatory relationship between p53 and ITM2A.
  • To explore the functional consequences of this interaction in cancer.

Main Methods:

  • Western blotting and quantitative PCR to assess protein and mRNA levels.
  • Immunoprecipitation to confirm physical interaction.
  • Phosphorylation site analysis and subcellular localization studies.
  • Reporter assays to measure transcriptional activity.

Main Results:

  • p53 upregulates ITM2A expression, creating a negative feedback loop.
  • ITM2A expression is reduced in multiple tumor types, especially with mutant p53.
  • Low ITM2A correlates with decreased patient survival.
  • ITM2A modulates p53 phosphorylation, promotes cytoplasmic localization, and suppresses p53 transcriptional activity.
  • ITM2A depletion enhances p53 nuclear accumulation and activity.

Conclusions:

  • A novel p53-ITM2A autoregulatory circuit was discovered.
  • ITM2A acts as a negative regulator of p53.
  • ITM2A may serve as a prognostic marker and therapeutic target in cancers with p53 alterations.
Keywords:
ITM2Anegative feedbackp53phosphorylationtumor suppressor

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