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Published on: November 30, 2016
Wheat Germ Glycoprotein (WGPII) Alleviates Cyclophosphamide-Induced Intestinal Injury: Integrative Multiomics Reveals
Jinpeng Zhang1, Tiantian Zhao1, Fan Zhang1
1College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin300392, China.
None:
Wheat germ glycoprotein (WGPII) exhibits immunomodulatory activity; however, its protective mechanisms against intestinal injury remain unclear. This study investigated WGPII effects in cyclophosphamide (CTX)-induced intestinal injury mice. WGPII significantly reversed immunosuppression by increasing serum cytokines and immunoglobulins and mitigated intestinal damage by upregulating SIgA and tight junction proteins. 16S rDNA sequencing and metabolomics revealed that WGPII enriched beneficial taxa (Lachnospiraceae_NK4A136_group, Roseburia) and decreased Bacteroides, correlating with TCA cycle activation and key metabolite enrichment (α-ketoglutarate, pyruvate, and N-acetyl-l-glutamate). WGPII also upregulated colonic miR-217-5p, miR-769-x, and miR-654-3p, while suppressing their predicted targets (Cxcl12, Mapk4, IL-17F). Correlation analysis suggested a potential "miRNA-gut microbiota-metabolite" regulatory network that may contribute to WGPII's protective effects. These correlational findings provide a theoretical basis for developing WGPII-based functional products for intestinal health, though direct mechanistic validation is warranted.
