Technical note: Micromanipulation flicking technique for embryo biopsy in bovine embryos

Embryo biopsy is widely used in human assisted reproduction for preimplantation genetic testing, but its application in bovine in vitro fertilization (IVF) programs remains limited, largely due to the cost and complexity of laser-based systems. Here we describe a micromanipulation method for laser-free embryo biopsy in bovine embryos that relies on a mechanical separation technique to collect embryo samples while preserving embryo viability. This method provides a practical alternative for embryo biopsy in bovine IVF systems and may facilitate the incorporation of embryo genotyping into genetic improvement programs.