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Updated: Aug 21, 2026
12:55
Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation
Published on: September 29, 2019
Simple and fast production of biologically active CXCL12/SDF-1 isoforms in E. coli using a Nanobody solubility
Vanessa Wittek1, Jonas Kaynert1, Manuel M Vicente1,2
1Institute of Clinical Biochemistry, Hannover Medical School, Hannover 30625, Germany. Muenster.Anja@mh-hannover.de.
Chemical Communications (Cambridge, England)
|August 19, 2026
Abstract:
CXCL12 is commonly produced by laborious inclusion-body refolding approaches. Here, we present a fast, scalable, and low-cost method for producing biologically active CXCL12/SDF-1 isoforms in E. coli within two days using a nanobody solubility partner, enabling simple and fast in-house production of the chemokines for multiple research applications.
