Related Experiment Video
Updated: Aug 21, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
A Temporal Atlas of the Nuclear Androgen Receptor Proximal Interactome
None:
Androgen receptor-interacting proteins (AR-IPs) comprise nearly 1,000 cataloged partners, yet how AR engages this interactome inside the nucleus, in what temporal order, and through what molecular handoffs, remains uncharted. Here, we construct a minute-scale temporal atlas of the nuclear AR proximal interactome by proximity-labeling quantitative mass spectrometry (PL-qMS) in androgen-treated LNCaP prostate cancer cells, capturing 84.2% of the known AR-interactome and resolving 3,378 nuclear AR-proximal interacting proteins (AR-PIPs) across six time points. The atlas recapitulates the cyclic sequential recruitment model established at AR-regulated loci by classical ChIP and recovers 100% of previously known AR interactors from the Launonen 2021 ChIP-SICAP chromatome. Embedded within this canonical chromatin signature, we uncover a time-resolved translation-to-transcription handoff in which cap-binding eIF4G and 4E-BP1 are transiently AR-proximal at the earliest time points, verified by PLA. The atlas converts the AR coregulator catalog into a temporally resolved quantitative framework for AR-dependent transcription.
Synopsis:
A minute-scale temporal atlas of the nuclear AR-proximal interactome resolves a translation-to-transcription handoff during the androgen response. Temporal nuclear AR-proximal interactome atlas in androgen-treated LNCaP cells3,378 nuclear AR-PIPs recovered, 100% of previously known Launonen AR partnersCap-binding eIF4G and 4E-BP1 are transiently AR-proximal at early time pointsPLA verifies AR-eIF4G and AR-4E-BP1 proximal interactions pointing to a translation-to-transcription handoff.
Related Concept Videos
Transducer Mechanism: Nuclear Receptors
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Internal Receptors
Protein-protein Interfaces
Assembly of Signaling Complexes
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...

