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Comparative antioxidant efficacy of D-Cateslytin combined with modified triple antibiotic paste and calcium
Alen Romel1, Harish Shetty1, Vidhyadhara Shetty1
1Department of Conservative Dentistry and Endodontics, Yenepoya Dental College, Yenepoya (Deemed to be University), Mangalore, Karnataka, India.
Background:
Successful endodontic treatment depends on effective elimination of microorganisms and the creation of a favorable biological environment for periapical healing. Conventional intracanal medicaments such as calcium hydroxide and Modified Triple Antibiotic Paste (MTAP) possess antimicrobial activity; however, their ability to modulate oxidative stress within the root canal environment is limited. D-Cateslytin, a peptide derived from Chromogranin A, has shown promising antimicrobial activity against various oral pathogens and may also contribute to antioxidant effects when used in combination with traditional intracanal medicaments.
Aim:
To evaluate and compare the antioxidant activity of D-Cateslytin in combination with calcium hydroxide and MTAP.
Materials And Methods:
An in vitro experimental study was conducted using six experimental groups: control, D-Cateslytin, calcium hydroxide, MTAP, D-Cateslytin + calcium hydroxide, and D-Cateslytin + MTAP. The antioxidant activity was assessed by performing 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging activity and nitric oxide (NO) scavenging activity. The absorbance was measured at 517 nm for DPPH and 540 nm for NO.
Statistical Analysis:
Data were analysed using one-way ANOVA followed by Bonferroni post hoc test (p < 0.05).
Results:
Both D-Cateslytin combination groups demonstrated greater antioxidant activity than calcium hydroxide and MTAP alone. Although the D-Cateslytin + MTAP group exhibited the highest antioxidant activity, it was not significantly different from D-Cateslytin alone or D-Cateslytin + calcium hydroxide.
Conclusion:
D-Cateslytin exhibited superior antioxidant activity compared to conventional intracanal medicaments. The addition of D-Cateslytin to calcium hydroxide and MTAP may enhance the biological properties of intracanal medicaments and could contribute to improved endodontic treatment outcomes.