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Updated: Aug 21, 2026

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
10:40

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

Screening and functional analysis of a host membrane protein interacting with the structural protein VP1 of deformed

Di Liu1,2, Yuting Xiao1,2, Xu Li1,2

  • 1Collaborative Innovation Center for Zoonosis Prevention and Treatment, Jinzhou Medical University, Jinzhou, China.

Frontiers in Microbiology
|August 20, 2026
PubMed
Abstract

Insights

Deformed wing virus (DWV) structural protein VP1 interacts with honeybee NCR-like G-protein-coupled receptor. This interaction impacts antimicrobial peptide expression and influences DWV replication, offering insights into honeybee viral pathogenesis.

Area of Science:

  • Virology
  • Insect Pathology
  • Molecular Biology

Background:

  • Deformed wing virus (DWV) significantly impacts Western honeybee (Apis mellifera) populations.
  • Understanding host-pathogen interactions at the molecular level is crucial for bee health.
  • The specific mechanisms of DWV infection, particularly protein interactions, are not well-defined.

Purpose of the Study:

  • To identify honeybee host proteins interacting with the DWV structural protein VP1.
  • To functionally characterize the interaction between DWV VP1 and identified host proteins.
  • To elucidate the role of this interaction in honeybee immunity and viral replication.

Main Methods:

  • Yeast membrane protein two-hybrid system for screening host-viral protein interactions.
  • Glutathione S-transferase (GST) pull-down and co-immunoprecipitation (Co-IP) for interaction validation.
  • RNA interference (RNAi) to assess gene function and viral replication.

Main Results:

  • Identified a neuropeptide capa (NCR) like G-protein-coupled receptor interacting with DWV VP1.
  • Confirmed the interaction using GST pull-down and Co-IP assays.
  • RNAi knockdown of NCR-like in DWV-infected bees resulted in a sixfold increase in viral replication.

Conclusions:

  • DWV VP1 interacts with the honeybee NCR-like protein.
  • This interaction modulates the expression of antimicrobial peptides (AMPs).
  • The interaction plays a role in regulating DWV replication and viral pathogenesis in honeybees.
Keywords:
VP1 proteindeformed wing virusinteracting proteinsviral replicationyeast membrane protein two-hybrid system

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