Related Experiment Video
Updated: Aug 22, 2026

06:44
Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells
Published on: March 1, 2024
BRAF and MEK caught in flagrante delicto
Dong Man Jang1, Michael J Eck1
1Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
Molecular Cell
|August 20, 2026
Abstract
None:
Kondo et al. describe crystal structures of a heterotetrameric complex of BRAF and MEK that reveal a heretofore unseen asymmetric interaction of the N-terminal acidic motif in the BRAF dimer and capture BRAF in the act of phosphorylating MEK.
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