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Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery
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Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery

Published on: October 4, 2024

Tudor staphylococcal nuclease promotes Bombyx mori cytoplasmic polyhedrosis virus replication through lipid metabolic

Mian Muhammad Awais1,2, Nasir Mehmood1, Wensheng Yu1

  • 1Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.

Insect Science
|August 21, 2026
PubMed

Insights

Tudor staphylococcal nuclease (TSN) is a key host factor for Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) replication. This study reveals a virus-induced axis linking host lipid metabolism to viral replication via BmTSN and BmSREBP1.

Area of Science:

  • Virology
  • Molecular Biology
  • Insect Pathology

Background:

  • Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) causes significant economic losses in sericulture.
  • Understanding host factors supporting BmCPV replication is crucial for disease management.

Purpose of the Study:

  • To identify and characterize host factors involved in BmCPV replication in silkworms.
  • To elucidate the mechanism by which these host factors influence viral propagation.

Main Methods:

  • RNA interference (RNAi) for gene knockdown and overexpression studies in BmN cells.
  • Quantitative analysis of viral replication and gene expression.
  • Analysis of lipid metabolism and gene regulatory networks.

Main Results:

  • Tudor staphylococcal nuclease (TSN) was identified as a critical pro-viral host factor, with its expression upregulated upon BmCPV infection.
  • BmTSN knockdown suppressed viral replication, while overexpression enhanced it.
  • BmTSN promotes viral replication by enhancing lipid accumulation through the regulation of BmSREBP1 and other lipid-related genes.
  • A regulatory loop was identified where BmSREBP1 activates BmTSN expression, linking host lipid metabolism to BmCPV replication.

Conclusions:

  • The study identifies a virus-induced BmTSN-BmSREBP1 axis that connects host lipid metabolism to BmCPV replication.
  • BmTSN is a key host-dependency factor for BmCPV, offering potential targets for controlling silkworm diseases.
Keywords:
BmCPVBmSREBP1BmTSNBombyx morihost–virus interactions

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