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Downregulation of Lgals3 Alleviates Inflammatory Response and Apoptosis in a Mouse Model of Cerebral
Zhaodan Gan1, Haifang Lai2, Yun Yang1
1Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Purpose:
The present work investigated the expression and function of Lgals3 in cerebral ischemia/reperfusion (cerebral IR) injury.
Methods:
Differentially expressed genes in cerebral IR were analyzed by the Gene Expression Omnibus (GEO) microarray. A middle cerebral artery occlusion (MCAO) mouse model was constructed. Adeno-associated virus serotype 9 vector carrying Lgals3 shRNA was administered to mice via tail vein injection. Four weeks after virus administration, mice were subjected to MCAO modeling. Neurological function was reflected by evaluating the neurological deficit score. To assess infarct size and neuronal apoptosis, triphenyl tetrazolium chloride (TTC) staining and TUNEL assay were performed. The concentrations of IL-6, TNF-α, IL-4, and IL-1β in brain tissues were measured using enzyme-linked immunosorbent assay (ELISA). Co-immunoprecipitation (Co-IP) was performed to examine the interaction between Lgals3 and TLR4. In addition, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting were employed to analyze gene and protein expression levels.
Results:
GEO bioinformatics analysis revealed that Lgals3 expression was significantly upregulated in cerebral IR. MCAO mouse model was successfully established, and Lgals3 was markedly upregulated in brain tissues of MCAO mice. Lgals3 knockdown reduced infarct volume, improved neurological deficit scores, and inhibited neuronal apoptosis, accompanied by decreased levels of pro-inflammatory cytokines (IL-6, TNF-α, and IL-1β) and increased levels of anti-inflammatory cytokine IL-4 in brain tissues of MCAO mice. Lgals3 silencing inhibited the activation of the TLR4/MyD88/NF-κB pathway, as evidenced by downregulated expression of TLR4, MyD88, p-p65, and p65. Co-IP experiment confirmed a direct interaction between Lgals3 and TLR4.
Conclusion:
Lgals3 knockdown alleviates neuroinflammation and neuronal apoptosis in brain tissues of mice with cerebral IR injury. The present findings indicate that Lgals3 participates in the progression of cerebral IR injury, and may serve as a potential intervention target for ameliorating cerebral IR damage.
Insights
Lgals3 (lectin, galactoside-binding, soluble, 3) knockdown reduces brain damage and inflammation after stroke in mice. This suggests Lgals3 is a potential therapeutic target for cerebral ischemia/reperfusion injury.
Area of Science:
- Neuroscience
- Immunology
- Molecular Biology
Background:
- Cerebral ischemia/reperfusion (IR) injury is a significant cause of neurological damage.
- The role of Lgals3 (lectin, galactoside-binding, soluble, 3) in cerebral IR injury remains unclear.
Purpose of the Study:
- To investigate the expression and function of Lgals3 in cerebral IR injury.
- To explore the potential of Lgals3 as a therapeutic target for cerebral IR.
Main Methods:
- Gene expression analysis using GEO microarray and bioinformatics.
- Establishment of a middle cerebral artery occlusion (MCAO) mouse model.
- Lgals3 knockdown using adeno-associated virus serotype 9 vector.
- Assessment of neurological function, infarct volume, and neuronal apoptosis.
- Measurement of inflammatory cytokines via ELISA.
- Analysis of TLR4/MyD88/NF-κB pathway activation using Co-IP, qRT-PCR, and Western blotting.
Main Results:
- Lgals3 expression was significantly upregulated in cerebral IR.
- Lgals3 knockdown reduced infarct volume, improved neurological scores, and inhibited neuronal apoptosis.
- Lgals3 silencing decreased pro-inflammatory cytokines (IL-6, TNF-α, IL-1β) and increased anti-inflammatory cytokine (IL-4).
- Lgals3 knockdown inhibited the TLR4/MyD88/NF-κB pathway activation.
- A direct interaction between Lgals3 and TLR4 was confirmed.
Conclusions:
- Lgals3 knockdown alleviates neuroinflammation and neuronal apoptosis in cerebral IR injury.
- Lgals3 plays a role in the progression of cerebral IR injury.
- Lgals3 represents a potential therapeutic target for mitigating cerebral IR damage.