Related Experiment Video
Updated: Aug 22, 2026

Characterization and Functional Prediction of Bacteria in Ovarian Tissues
Published on: October 23, 2021
Intestinal microbiota alteration in women with adenomyosis and clinical characteristics correlation
Yujing Guo1, Hua Duan1, Fang Chen1
1Department of Minimally Invasive Gynecology, Beijing Obstetrics and Gynecology Hospital, Beijing Maternal and Child Health Care Hospital, Capital Medical University, Beijing, China.
Introduction:
Adenomyosis is an enigmatic disease with estrogen dependence, and the exact pathogenic mechanisms remain confusing. Limited evidence points out the presence of gut flora changes in adenomyosis. However, evidence on how adenomyosis and gut microbiota crosstalk remains insufficient.
Methods:
This study included 30 female diagnosed with adenomyosis and 28 healthy control participants. Fecal samples were collected from all subjects prior to the initiation of pharmacological treatment. The 16S rRNA gene sequencing technique was utilized to analyze the fecal samples, with the objective of characterizing the composition and potential functional profile of the gut microbiota, as well as examining the correlations between the gut microbiota and disease-related clinical variables.
Results:
Compared with healthy controls, patients with adenomyosis showed a distinct gut microbial community composition. The adenomyosis group was significantly enriched in Prevotella_9 and Ligilactobacillus, whereas the control group had an enrichment in Anaerofustis, Akkermansia, and Anaerostipes. PICRUSt2 results showed significant down-regulation of PWY0-1061 (superpathway of L-alanine biosynthesis), P184-PWY (protocatechuate degradation I (meta-cleavage pathway)), and DHGLUCONATE-PYR-CAT-PWY (glucose degradation (oxidative)) pathways in the ADS group. Correlation analysis further revealed that the relative abundances of Akkermansia, Roseburia, Dialister, and Lachnoclostridium were significantly associated with clinical features of the disease.
Conclusion:
A potential association exists between adenomyosis and the intestinal microbiome. Clinical characteristics of adenomyosis are related to the intestinal microbiota. These findings offer microbiota-derived evidence and new perspectives on the pathophysiology of adenomyosis.
Related Concept Videos
Dysbiosis of the Gut Microbiota
Microbiota of the Urogenital Tract
Development of Human Microbiota
Microbiota Modulation by Antibiotics
Introduction to the Human Microbiota
Oogenesis