Related Experiment Video

Updated: Aug 22, 2026

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

Molecular Characterization of Rotavirus From Under-Five Children Presenting With Diarrhea at a Tertiary Hospital in

Kellu Bukar Ali1, Yahaya Yaqub2, Mairo Usman Kadaura3

  • 1Department of Medical Microbiology, University of Maiduguri, Maiduguri, Borno State, Nigeria, unimaid.edu.ng.

International Journal of Pediatrics
|August 21, 2026
PubMed

Insights

Rotavirus gastroenteritis (RVGE) is prevalent in Nigerian children under 5, with G9P[6] being the most common genotype. Evaluating rotavirus vaccine efficacy is crucial to reduce childhood deaths from this diarrheal disease.

Area of Science:

  • Pediatrics
  • Virology
  • Public Health

Background:

  • Diarrhea poses a significant threat to child health in developing nations.
  • Rotavirus is a primary cause of severe, potentially fatal, acute watery diarrhea in young children.
  • Understanding rotavirus gastroenteritis (RVGE) prevalence and genotypes is vital for effective control.

Purpose of the Study:

  • To determine the prevalence of rotavirus gastroenteritis (RVGE) in children under five years of age.
  • To characterize the circulating rotavirus genotypes (G and P types) in the study population.
  • To inform public health strategies and vaccine efficacy evaluations in Nigeria.

Main Methods:

  • A cross-sectional study involving 173 children with acute diarrhea was conducted.
  • Stool samples were screened for rotavirus antigen using lateral flow immunochromatography.
  • VP7 (G type) and VP4 (P type) genotypes were identified using reverse transcription-polymerase chain reaction (RT-PCR).

Main Results:

  • Rotavirus prevalence was 28% by immunochromatography and 14.5% by RT-PCR.
  • Identified G types included G1, G2, G1G2, G9, G12, and G nontypeable (G NT).
  • Identified P types included P4, P6, and P8, with G9P[6] being the most frequent genotype combination (28%).

Conclusions:

  • A high prevalence of RVGE was observed in Northeast Nigeria.
  • The study identified key circulating rotavirus genotypes, including G9P[6].
  • Evaluating the efficacy of the current rotavirus vaccine (ROTAVAC) is recommended to decrease RVGE-associated mortality.
Abstract

Keywords:
NigeriaRT-PCRdiarrhearotavirusunder-five childrenvaccine-preventable diseases

More Related Videos

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR15:16

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Published on: July 22, 2012

Related Concept Videos

Poliomyelitis01:17

Poliomyelitis

Poliomyelitis is caused by poliovirus, a small, non-enveloped, positive-sense RNA virus of the Picornaviridae family and Enterovirus genus. Transmission occurs primarily via the fecal-oral route, often through ingestion of contaminated water or food. The virus initially replicates in the oropharynx and intestinal mucosa, particularly in lymphoid tissues such as the tonsils, Peyer’s patches, and regional lymph nodes. Primary viremia follows, allowing dissemination throughout the body.In most...
Bacterial Gastroenteritis01:18

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...