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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Clavukellines A-N: dolabellane diterpenes from the soft coral Clavulariakoellikeri
Zhongyu Lu1, Xiao Zhu1, Xin Zhou1
1Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003,China.
Abstract:
Chemical investigation of the soft coral Clavularia koellikeri led to the isolation of fourteen new diterpenoid derivatives clavukellines A-N (1-14) featuring the dolabellane-type skeleton. Structural characterization of these compounds was accomplished through an integrated analytical strategy combining NMR spectroscopy and HR-ESI-MS. Absolute configurations were unambiguously assigned using ECD spectral simulation and DP4 + probability analysis. Preliminary pharmacological evaluation indicated that compounds 8 and 13 exhibited anti-inflammatory activity, while compounds 1-14 showed antithrombotic activity. This study reveals promising anti-inflammatory and antithrombotic potentials that warrant further investigation.
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