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Published on: October 31, 2025
Silent Circulation of Spotted Fever Group - Like Rickettsia Among Asymptomatic Border Personnel in the Eastern
Sauvik Dasgupta1, Sarthak Dasgupta1, Stanzin Dolkar1
1Zoological Survey of India, Prani Vigyan Bhavan, Kolkata, West Bengal, India.
Abstract:
Rickettsial infections are usually investigated in febrile patients, whereas molecular evidence from clinically asymptomatic populations remains limited. In this study, we screened 50 apparently healthy adult male Indo-Tibetan Border Police personnel stationed in Dirang, West Kameng district, Arunachal Pradesh, during December 2025. Of the 50 individuals, 27 (54.0%) were positive by nested gltA PCR and by TaqMan real-time PCR. A subset of 15/27 (55.6%) were additionally positive for 17 kDa. None reported fever, rash, eschar, lymphadenopathy, or hospitalization around sampling and/or during follow-ups. Sequencing placed the gltA and 17 kDa amplicons within spotted fever group rickettsiae, with affinity to R. hoogstraalii/R. asemboensis lineage. These findings provide multi-targeted molecular evidence of spotted fever group (SFG) like Rickettsia in clinically asymptomatic border personnel and highlight the need for serology, longitudinal follow-up, and vector surveillance to clarify its epidemiological significance.
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