Related Experiment Video
Updated: Aug 26, 2026

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
Published on: September 2, 2019
Enzyme-linked chaperone assay: a novel bacterial detection method for viability discrimination
Rachel Herschlag1, Carolee T Bull2
1Department of Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, PA 16892, USA; One Health Microbiome Center, The Pennsylvania State University, University Park, PA 16892, USA.
Abstract:
This study introduces enzyme-linked chaperone assay (ELCA), a novel antibody-free biosensor targeting Pseudomonas syringae effector-chaperone interactions for diagnostic purposes. Validated via a linear mixed effects model across three independent experiments, ELCA achieved a 102 CFU/mL limit of detection, successfully discriminating live target cells from dead populations and non-target strains.

