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Updated: Aug 27, 2026
Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
Published on: October 8, 2021
Aptamer-functionalized aggregation-induced emission micelles for integrated detection and photodegradation of
Xingjie Wu1,2, Guihua Zhu3,4, Qianqian Guo3,4
1State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, School of Pharmaceutical Sciences, Guizhou Medical University, No.6 Ankang Avenue, Guian New District, Guiyang, 561113, Guizhou Province, P. R. China. wxj_gmu@126.com.
Abstract:
Lipopolysaccharide (LPS) contamination in biopharmaceuticals poses severe clinical risks, yet its detection and removal remain enduring challenges. Conventional detection methods are frequently hindered by low endotoxin recovery. Established clearance techniques often compromise the integrity of sensitive biologics or introduce secondary contaminants. To overcome these constraints, we developed an integrated platform for simultaneous LPS detection and removal. This system employs aptamer-conjugated micelles that encapsulate an aggregation-induced emission photosensitizer with high reactive oxygen species quantum yield. The surface aptamers facilitate specific LPS capture, followed by efficient light-triggered oxidative degradation. The capture-first design concentrates LPS near the photosensitizer-loaded core to maximize degradation efficiency. When validated in endotoxin-contaminated insulin solutions, the platform markedly reduced the ELISA-detectable LPS signal under the tested conditions, without measurably impairing insulin-induced glucose uptake in 3T3-L1 adipocytes in this preliminary assay. This biocompatible strategy bridges the gap between LPS monitoring and safe decontamination.
