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Evaluation of the stability of endoscope samples
Lionel Pineau1, Vanessa Cordier2
1Eurofins Biotech-Germande, Aix en Provence, France.
Background:
Endoscope-related infections remain a concern despite improved reprocessing protocols. Transport conditions between sampling and analysis may affect sample reliability significantly, yet current guidelines remain imprecise.
Aim:
To evaluate the stability of endoscope samples collected using a pharmacopoeia diluent neutralizer with sodium thiosulfate (PND + thio) stored at 5 °C ± 3 °C, and to determine the maximum acceptable time before microbiological analysis.
Methods:
Artificially contaminated endoscope samples were prepared using four indicator micro-organisms (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans). Samples were stored at 5 °C ± 3 °C and analysed at predefined time points up to 72 h. Microbial counts were compared with initial values using statistical analysis and ISO 11133 acceptance criteria.
Findings:
Microbial concentrations remained stable or within acceptable variation ranges up to 48 h for all tested micro-organisms. The presence of neutralizing and surfactant agents appeared to preserve microbial viability and counteract disinfectant residues.
Conclusion:
Endoscope samples collected with PND + thio can be stored for up to 48 h at 5 °C ± 3 °C without compromising microbiological analysis, supporting more flexible and sustainable transport conditions.
