Seasonal Water Level Fluctuations Mediate Hydrological Connectivity and Shape Zooplankton Metacommunity in Poyang...

Poyang Lake is a typical floodplain lake hydrologically linked to the middle-lower Yangtze River. Its peripheral saucer-shaped sub-lakes undergo seasonal hydrological alternation between isolation and connectivity driven by annual water level fluctuations. To date, the coupling between seasonal hydrological rhythms and zooplankton metacommunity assembly in these saucer floodplain lakes remains poorly understood. In this study, four representative saucer sub-lakes within the Poyang Lake National Nature Reserve (Banghu, Zhonghuchi, Shahu and Dahuchi) were selected as research objects. Seasonal quantitative zooplankton surveys and synchronous hydro-physicochemical monitoring were conducted across four hydrological phases from 2012 to 2013: low-water isolation (spring), high-water connectivity (summer), water recession transition (autumn), and extreme low-water closure (winter). We systematically explored how water level fluctuations regulate zooplankton metacommunity assembly processes. In total, 95 zooplankton species were identified, among which rotifers (68 species) constituted the absolute dominant taxon, and 15 species were identified as year-round absolute dominants exhibiting regular seasonal succession in response to hydrological shifts. Our results demonstrate that hydrological connectivity plays a strong role in regulating the relative strength of species dispersal and environmental filtering. During spring and winter, complete hydrological isolation blocked inter-lake species exchange, making local environmental filtering the main driver of metacommunity assembly. Isolated sub-lakes harbored fewer total species but abundant endemic taxa, accompanied by pronounced spatial community heterogeneity. When water levels exceeded the 17.3 m threshold in summer, full water exchange between sub-lakes and the main lake coincided with widespread species dispersal, which appeared to represent the primary assembly process. The four sub-lakes shared 23 co-occurring species during this period, with highly homogenized community structures and minimal spatial differentiation (Global test: R = 0.47, p = 0.001). In autumn, receding water levels weakened inter-lake connectivity, such that environmental filtering and dispersal jointly structured zooplankton metacommunities; significant spatiotemporal differences were detected in zooplankton density, biomass and α-diversity (p < 0.05). Critical hydro-physicochemical factors of environmental filtering included water temperature, chlorophyll a, pH, electrical conductivity and turbidity, yet the dominant limiting factor varied spatially across sub-lakes due to divergent basin topography and water residence time. Species co-occurrence network analysis revealed that zooplankton communities achieved the highest stability during the fully connected summer phase. In early autumn water drawdown, species association networks became fragmented, and community stability reached its annual minimum. This study elucidates the mechanistic link between floodplain lake hydrological rhythms and zooplankton metacommunity assembly, providing theoretical support and baseline data for wetland biodiversity conservation and ecosystem management of Poyang Lake floodplains.